site directed mutagenesis

A Novel Method for Site-directed Mutagenesis and Its Application in DdsA Mutant Research

一种新型的基因定点突变方法及其在DdsA突变研究中的应用

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Unnatural amino acid site-directed mutagenesis was applied to adenylate kinase to study its mechanism.

非天然氨基酸定点突变成功地应用在腺苷酸激酶上来研究其作用机理.

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Site-directed mutagenesis can correctly change the base sequence and get mutant proteins.

基因定点突变技术可以精确地改变基因的特定碱基序列,获得突变蛋白质.

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In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.

过去主要是通过亲和标记和点突变等实验方法进行研究。

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The mutant G138P was obtained by in vitro site directed mutagenesis of GI gene.

用双引物法对GI基因进行体外定点突变，构建了GI突变体G138P。

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For further exploring and studying the expression and regulation of nif genes, we carried out site-directed mutagenesis on a crucial tyrosine residue of the positive regulator protein NifA.

在全基因组测序和芯片分析的基础上，为进一步探讨和研究A1501固氮基因的转录调控机制，本研究开展了正调控蛋白NifA一个关键位点的定点突变分析。

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Using recombinant glucagon expression system, the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis. Glucagon gene mutation was testified by DNA sequencing.

在获得重组胰高血糖素基因工程菌基础上，利用定点突变技术改造其第21位氨基酸天冬氨酸为丙氨酸，并经DNA测序证明胰高血糖素基因发生了点突变。

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Changes of Glucagon Structure and Function by~ ( 21) Ala Site-Directed Mutagenesis

~（21）Ala定点突变胰高血糖素及结构与功能变化

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Study on Active Site Residues of Human Brain Hexokinase ⅰ by Site directed Mutagenesis

用定位突变方法对人脑己糖激酶活性位点的研究

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Methods: Glutamine residue in 219 of trichosanthin was mutated into cysteine residue ( Q219C) by site-directed mutagenesis. The mutein was then modified by polyethylene glycol ( PEG). Ribosome-inactivating activity and abortifacient activity of the modified products were determined.

方法：利用定点突变将TCS第219位的天冬酰胺残基改变为半胱氨酸残基（Q219C）以供定点聚乙二醇（PEG）修饰，测定修饰产物的致核糖体失活活性及小鼠中期引产活性。

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Moreover, results of the site-directed mutagenesis of a C-repeat/ DRE and two APE-like motifs in a promoter-like region(– 630/ – 308) suggested that two APE-like motifs might be essential for transcriptional regulation of the bkt gene.

对其启动子类似区（-630/-308）中两种功能元件的定点突变结果发现APE-like（蓝光调控元件）对于bkt基因自身的转录可能存在影响。

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